Translational_Unit
rbs+ftsK

Part:BBa_I742164:Design

Designed by: Caroline Dahl and Xiaonan Wang   Group: iGEM07_Edinburgh   (2007-10-25)


ftsK (cell division protein) of E. coli with native rbs


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1192
    Illegal PstI site found at 1255
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 755
    Illegal PstI site found at 1192
    Illegal PstI site found at 1255
    Illegal NotI site found at 79
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3821
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1192
    Illegal PstI site found at 1255
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1192
    Illegal PstI site found at 1255
    Illegal AgeI site found at 561
    Illegal AgeI site found at 1307
    Illegal AgeI site found at 1583
    Illegal AgeI site found at 2258
    Illegal AgeI site found at 2375
    Illegal AgeI site found at 2414
    Illegal AgeI site found at 3003
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1335
    Illegal SapI.rc site found at 3429


Design Notes

Note that this is the native sequence with a TTG start codon and two internal PstI sites.


Source

Escherichia coli JM109 (a K12 strain).

References